Estimation of some Phenolic compound and Antimicrobial activity of Green Tea (Camellia sinensis L.) extract

In this article, the activity of antibacterial of methanolic owns a green tea leaves (Camellia sinensis) extracted on different pathogenic strains has been investigated with the use of paper disc diffusion technique. the tested strains were Enteroccous faecalis , Escherichia coli , Staphylococcus aureus ,Methicillin –resistant staphylococcus aureus (MRSA) and pseudomonas aeruginosa. The results were showed that the extract produced inhibition zones in all tested bacteria ranging from 18 mm in S.aureus to 12mm in p.aeruginosa . we can concluded that The extract from green tea leaves may be helpful in the fight against new drug resistance brought on by the germs mentioned above. Some phenolic materials were determined using a high-performance liquid chromatograph(hplc), and three extraction methods (aqueous, methanol and ethanol) were used. The following phenolic substances were estimated) Epicatechin, Gallic acid, Chlorogenic, Caffeine, Quercetin, Kaempferol). The methanol extraction method significantly outperformed the other of the methods and recorded the highest averages of ( Epicatechin, Gallic acid, Chlorogenic, Caffeine, Quercetin, Kaempferol) reached) 30.367, 26.467, 37.533, 34.800, 25.533, 42.700) mg.g sequentially.

Anahtar Kelimeler: Antimicrobial activity, green tea extract, paper disc diffusion

In this article, the activity of antibacterial of methanolic owns a green tea leaves (Camellia sinensis) extracted on different pathogenic strains has been investigated with the use of paper disc diffusion technique. the tested strains were Enteroccous faecalis , Escherichia coli , Staphylococcus aureus ,Methicillin –resistant staphylococcus aureus (MRSA) and pseudomonas aeruginosa. The results were showed that the extract produced inhibition zones in all tested bacteria ranging from 18 mm in S.aureus to 12mm in p.aeruginosa . we can concluded that The extract from green tea leaves may be helpful in the fight against new drug resistance brought on by the germs mentioned above. Some phenolic materials were determined using a high-performance liquid chromatograph(hplc), and three extraction methods (aqueous, methanol and ethanol) were used. The following phenolic substances were estimated) Epicatechin, Gallic acid, Chlorogenic, Caffeine, Quercetin, Kaempferol). The methanol extraction method significantly outperformed the other of the methods and recorded the highest averages of ( Epicatechin, Gallic acid, Chlorogenic, Caffeine, Quercetin, Kaempferol) reached) 30.367, 26.467, 37.533, 34.800, 25.533, 42.700) mg.g sequentially.

Keywords: Antimicrobial activity, green tea extract, paper disc diffusion

Sayfa Aralığı: 109-116

Hussein, M. H., Al-Khazraji, S. M., Hassoon, A. S. & Kadhim, A. A. (2023). Estimation of some Phenolic compound and Antimicrobial activity of Green Tea (Camellia sinensis L.) extract. Journal of Current Research on Engineering, Science and Technology, 9 (1), 109-116.