Yıl:2022   Cilt: 8   Sayı: 2   Alan: Ziraat, Orman ve Su Ürünleri Temel Alanı

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Uoropathogenic Escherichia coli chromosomal study of cnf1 and fyuA genes among patients of urinary tract infection in Kirkuk city

Karwan Azeez MOHAMMAD ORCID Icon,Ibraheem Salih ALJEBORY ORCID Icon

This research has taken place at Kirkuk university/college of science/department of biology, Kirkuk hospital, pediatric hospital, and Azadi teaching hospital, in Kirkuk city, Iraq. Around 150 urine samples were taken from different ages and Patients of both genders with infections of the urinary tract according to symptoms and manifestations diagnosed by the examining physician then, these samples were investigated by a bacterial cell and an optical microscope detection next to heavy pus formation was clear evidence of harmful bacteria’s existence. Positive samples were cultivated and stored for genetic research by conventional PCR. A total of 93 urine samples contained positive pathogenic bacteria cultures, with 51 of them showing E. coli growth, out of 51 patients, 37 (72.5%) had Cystitis, whereas 14 (27.4%) had Pyelonephritis. The isolates were identified using API 20E, and this investigation revealed that females were infected at a higher rate than men, with 39 isolates (76.4%) infected compared to 12 isolates (23.5%). E. coli chromosomal DNA was extracted using a gene-aid DNA isolation kit. Genetic pattern occurrence of fyuA and cnf1 genes were investigated using conventional PCR, fyuA gene was found in all 51 (100%) isolates and also this study yielded that only 13 (25.4) isolates have carried cnf1 gene of which 12 isolates from Cystitis and only 1 isolate from Pyelonephritis. We conclude that iron uptake is crucial for UPEC which makes a relationship between the gene fyuA and UTI, The strains isolated from Cystitis show the greatest diversity of genes pattern than strains isolated from pyelonephritis, cytotoxic necrotizing factor 1 toxin encoded by cnf1 gene occur more likely in Cystitis.

Anahtar Kelimeler: UPEC, cnf1, fyuA, Cystitis, Pyelonephritis


Uoropathogenic Escherichia coli chromosomal study of cnf1 and fyuA genes among patients of urinary tract infection in Kirkuk city

This research has taken place at Kirkuk university/college of science/department of biology, Kirkuk hospital, pediatric hospital, and Azadi teaching hospital, in Kirkuk city, Iraq. Around 150 urine samples were taken from different ages and Patients of both genders with infections of the urinary tract according to symptoms and manifestations diagnosed by the examining physician then, these samples were investigated by a bacterial cell and an optical microscope detection next to heavy pus formation was clear evidence of harmful bacteria’s existence. Positive samples were cultivated and stored for genetic research by conventional PCR. A total of 93 urine samples contained positive pathogenic bacteria cultures, with 51 of them showing E. coli growth, out of 51 patients, 37 (72.5%) had Cystitis, whereas 14 (27.4%) had Pyelonephritis. The isolates were identified using API 20E, and this investigation revealed that females were infected at a higher rate than men, with 39 isolates (76.4%) infected compared to 12 isolates (23.5%). E. coli chromosomal DNA was extracted using a gene-aid DNA isolation kit. Genetic pattern occurrence of fyuA and cnf1 genes were investigated using conventional PCR, fyuA gene was found in all 51 (100%) isolates and also this study yielded that only 13 (25.4) isolates have carried cnf1 gene of which 12 isolates from Cystitis and only 1 isolate from Pyelonephritis. We conclude that iron uptake is crucial for UPEC which makes a relationship between the gene fyuA and UTI, The strains isolated from Cystitis show the greatest diversity of genes pattern than strains isolated from pyelonephritis, cytotoxic necrotizing factor 1 toxin encoded by cnf1 gene occur more likely in Cystitis.

Keywords: UPEC, cnf1, fyuA, Cystitis, Pyelonephritis


Atıf İçin

Mohammad, K. A. & Aljebory, I. S. (2022). Uoropathogenic Escherichia coli chromosomal study of cnf1 and fyuA genes among patients of urinary tract infection in Kirkuk city. Journal of Current Research on Engineering, Science and Technology, 8 (2), 193-202.


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